Xueba’s medical black technology system

Chapter 226 Mice that died of stress

stress.

Refers to individual non-specific reactions caused by various sources of stress.

It manifests as sympathetic nerve excitement, increased secretion of pituitary and adrenocortical hormones, increased blood sugar, increased blood pressure, accelerated heart rate, and accelerated breathing.

To take the simplest example, if someone is suddenly startled from behind, people will have various nervous reactions, or even more serious, they will be stunned or scared to death!
This is stress.

As early as 1974, physiologist Selye had studied stress response. In his research, he showed that long-term stress will cause a lot of damage to the body and even lead to body failure.

After all, from a normal perspective, if a person is worried and frightened all day long, how can he be in good health?
Therefore, it is not outrageous that stress leads to death.

There are many factors that cause stress.

Environment, temperature, sound, air, etc., various factors may become stressors.

Wang Wentao asked curiously: "Senior brother, how do you know?"

"It depends on the feed intake." Lu Liang replied.

His judgment method is very simple. Judging from his current recorded feed intake data, the feed intake of these two mice has been pitifully low since they were sent by the biological company.

After all, under stress, the hypothalamic parasympathetic nerves that regulate appetite are suppressed, and appetite is also in a suppressed state. How can there be appetite?

Lu Liang estimated that the cause of stress in these two mice was probably changes in the environment.

In human terms, it means he is not accustomed to the local environment.

Wang Wentao clicked his tongue and said, "Why does this thing die so easily?"

"Otherwise, do you think they are as tenacious as the rats in the sewers?" Lu Liang rolled his eyes and said.

However, this also made Lu Liang relax a little.

Mice are stressed to death, and this cause of death is related to individual differences in mice. After all, the physiques of mice cannot be generalized.

Of course, the most important thing is that this means that the death of the mouse was not caused by a problem in any part of his experimental plan, and the experiment can still continue.

Lu Liang said: "There are three spare mice in each group. The data of the dead mice will be eliminated and the data of the spare mice will be used to top up."

The spare mouse comes in handy at this time.

However, although there are spare mice, what worries Lu Liang is that judging from the current recorded mouse feeding data, there are still several mice whose feed intake is not high and are still in a state of stress.

If he continues to die, there won't be enough spare mice.

Especially the experimental group with tp53 mutation, two died at once, and now only the last mouse is left as a spare.

If something like this happens again, the tp53 mutation test group will not even have 12 mice, and the test will not be universal, and this group of tests will be completely useless.

Therefore, no more surprises can happen next.

Lu Liang thought for a moment, and then said: "In the future, the ventilation time of the rat room should be set to 12 hours."

Temperature is an important factor in stress generation.

In the current winter, low indoor temperatures can easily cause cold stress in mice.

Reducing ventilation will certainly make the air quality in the mouse house worse, but it is the only way to reduce stress in mice as much as possible.

Fortunately, in the next few days, there were no problems in the experiment.

The remaining stressed mice gradually adapted to the environment, and their weight and feed intake gradually recovered, which made Lu Liang feel a little relieved.

However, Lu Liang did not relax.

Injecting drugs into the stomach, recording the mice's feed intake and weight changes, and checking the mice's abnormalities have become Lu Liang's daily work.

These tasks were maintained until the end of the experiment,

After completing the sampling, Lu Liang immediately contacted Manager Nie of the biological company and sent the samples for testing. He needed the most time now, and sending for testing was undoubtedly the fastest way to get results.

After doing all this, Lu Liang finally breathed a sigh of relief.

What he has to do now is wait for the results to come out.Hengfeng Biology.

Zhang Qingchi stretched hard, and then glanced at the time in the lower right corner of the computer. The time was now [-]:[-] pm.

I can get off work in 10 minutes.

This made Zhang Qingchi couldn't help but feel happy. He was always the happiest after get off work.

However, when Zhang Qingchi was thinking about these things, the door of the office was pushed open. Zhang Qingchi turned around and found that the person who walked in was Manager Nie.

This made Zhang Qingchi's heart suddenly thump, and a bad premonition emerged in his heart.

As expected, Manager Nie walked straight up to Zhang Qingchi and placed a printed document in front of Zhang Qingchi.

"Team Leader Zhang, can you please work overtime today?" Manager Nie showed a flattering smile on his face.

Zhang Qingchi's face suddenly darkened. He didn't mention working overtime earlier than later, but he waited until he only had 10 minutes to get off work!
I am really afraid of what will happen.

"No, I have something to do today, so I can't add it." Zhang Qingchi refused without hesitation.

Manager Nie could only bite the bullet and continue to speak: "Team Leader Zhang, there is nothing I can do. These samples have just been received. They are from the company's major customers. They are of high priority, and the data customers are eager to use them."

"What does it have to do with me?" Zhang Qingchi sneered. That was the company's big customer, not his.

After a moment of silence, Manager Nie said tentatively: "How about this? I'll give you 1.5 times your salary for overtime work. How about that?"

Zhang Qingchi snorted coldly: "Do I seem to be in need of this little money?"

Faced with Zhang Qingchi, who refused to take any advice, Manager Nie had no choice. He sighed and said, "Okay, I'll put the information here first. Team Leader Zhang, you must remember to do it tomorrow. The customer is very urgent."

After seeing Manager Nie relent, Zhang Qingchi felt relieved, as long as he didn't force him to work overtime.

However, this also made Zhang Qingchi a little curious. Whose sample was this that could make Manager Nie willing to pay overtime and beg them to work overtime.

After all, Manager Nie has always been stingy in the company. In order to save money for the company, he works overtime voluntarily. There is no such thing as overtime pay.

Zhang Qingchi picked up the document and flipped through it. The document was marked with various indicators that the sample needed to be tested.

CEA, CA199, CA724 are tumor markers?
It should be some animal experiments related to cancer, right?

Zhang Qingchi has been doing testing for at least a few years. He has tested all indicators, so he is also familiar with the meaning behind these indicators.

Zhang Qingchi continued to look at the indicators marked on the data that needed to be tested.

Blood amylase, blood sugar indicators.?

This made Zhang Qingchi a little confused. In an animal experiment related to cancer, why should the samples be tested for indicators such as amylase and blood sugar? Shouldn't these indicators be related to the pancreas?

Wait, isn’t this an animal experiment on pancreatic cancer?

Zhang Qingchi was not a fool. He reacted instantly and then thought of the recent pancreatic cancer drugs.

In order to be sure, Zhang Qingchi glanced at all the indicators that needed to be tested.

There are many sample testing indicators, and there is no need to send them for inspection. They can be completed by any laboratory in the university, but it will take a little longer, and the other party is also busy with them.

Obviously, for the other party, the purpose of sending for inspection is to get the sample data out in the shortest possible time.

Thinking of this, Zhang Qingchi probably had an idea.

If his guess is correct, this batch of samples is indeed urgent.

After hesitating for a moment, Zhang Qingchi seemed to have made a decision. He sighed, then picked up his mobile phone and dialed Manager Nie's number.

“Manager Nie, I can work overtime on this batch of samples, but I’ll have to double the overtime pay!”

Manager Nie on the other end of the phone hesitated for a moment, but gritted his teeth and agreed: "Okay, no problem."

After getting the promise, Zhang Qingchi breathed a long sigh of relief. He sorted out the information, and then rushed towards the laboratory without stopping.

(End of this chapter)