Xueba’s medical black technology system

Chapter 225 Something went wrong in the experiment.

After finishing his meal, Lu Liang got up and went to pay the bill.

The price of the steamed vegetable restaurant is not expensive. Four dishes for three people cost more than 40 yuan in total.

After paying the bill and saying hello to Huang Wei, Lu Liang took Wang Wentao and walked directly towards the rat room.

Lu Liang took out the key to the mouse room from his pocket. After opening the mouse room, Lu Liang glanced at the situation in the mouse room. The cardboard box containing the mice was placed in the corner of the room.

There are three cartons in total, each containing mice with different mutation models.

"let's start."

Lu Liang opened the computer placed in the mouse room and said that this computer was Lu Liang's notebook that had been eliminated before. In line with the principle of making the best use of everything, Lu Liang threw the computer in the mouse room to record some Daily data.

Weighed, numbered, ear-tagged, and then randomized.

Mice of each mutation model were divided into control and experimental groups.

When loading the mice, Wang Wentao laid bedding in the mouse cage.

Wang Wentao said: "The litter is almost gone again."

Generally, the bedding materials for mouse cages are some wood chips. This seemingly inconspicuous thing is not cheap.

A box of sterile litter weighs about five or six kilograms, and the price is probably a few hundred.

The most important thing is that this thing consumes a lot of money and needs to be replaced every few days. One box of litter may not last one trial cycle.

"Let's use ordinary dunnage from now on. A sack costs 60 yuan, so we can build it hard." Lu Liang said thoughtfully.

Anyway, their test was not so strict that it required a sterile environment, and the use of ordinary litter had little impact on the final results.

If you are doing experiments such as mouse intestinal flora transplantation, you may need to use sterile litter.

After everything was taken care of, the experiment officially began.

On the first day of the experiment, Lu Liang and Wang Wentao administered drugs to all the experimental mice as usual. They had done a lot of this work before, so they were very skilled, and the mice did not find any abnormalities.

However, Lu Liang still discovered something was wrong by recording the mice's daily feed intake.

Some mice had ridiculously low feed intakes.

Other mice can eat several grams of feed a day, but a few mice can barely eat 1g of feed at the end of the day.

However, with insufficient data, Lu Liang had no way to analyze what went wrong and could only continue to observe.

On the second day of the experiment, the mice were still normal.

However, on the third day of the experiment, something went wrong with the mice.

In the morning, not long after Lu Liang arrived at the study room, he received a call from Wang Wentao, saying that a mouse had died.

Three died in one night!

When he heard this, Lu Liang couldn't help but frown.

In the past, during his experiments, he would encounter mice dying, but most of the time, one died occasionally.

Three of them died this time, and this was the first time Lu Liang encountered them.

After hanging up the phone, Lu Liang hurried towards the rat room.

In the mouse room, Wang Wentao was checking the conditions of other mice one by one. After seeing Lu Liang coming, Wang Wentao pointed to the three mouse cages on the table and said, "Where are they?" Lu Liang pulled out a pair of mouse cages from the box next to him. He put on disposable rubber gloves, then opened the mouse cage and checked the three mice Wang Wentao said. These three mice were indeed dead.

Wang Wentao continued: "When I came here for inspection in the morning, these three mice were already dead, but the other mice were still normal and had no problems."

Lu Liang asked: "Which groups do these three mice belong to?"

Wang Wentao replied: "One died in the control group of the KRAS gene mutation group, and then two died in the tp53 mutation experimental group, that's all."

After Lu Liang listened to Wang Wentao's answer, he turned on his computer in the mouse room and searched for the corresponding daily data through the ear tag numbers on the mice's ears.

Among the three dead mice, two mice had extremely low feed intake, and Lu Liang probably guessed the reason.

However, the feed intake of the other dead mouse was normal, which made Lu Liang feel a little puzzled.

After hesitating for a moment, Lu Liang took out a pair of surgical scissors from the drawer, and then pulled out a pair of forceps.

After fixing the limbs of the dead mouse on the foam board, Lu Liang picked up the scissors and quickly dissected the mouse.

The scissors were cut along the midline of the abdomen of the mouse toward the chest cavity. After cutting the skin, there was a layer of peritoneum underneath. After carefully cutting the peritoneum, all the internal organs of the mouse were exposed.

The shape and texture of the organs were normal, except for the stomach, which was a little red and swollen.

Lu Liang carefully cut open the stomach part, and the chyme that had not yet had time to digest flowed out, and the smell filled the air for a while.

At that moment, Lu Liang felt as if his heavenly spirit cap had been lifted. The smell was like rotten meat mixed with herring soaked in a jar to ferment, and the strong disgusting smell was like two chopsticks inserted directly into Lu Liang's nostrils.

Although Lu Liang had long been accustomed to the ammonia smell in the rat room, this smell still made Lu Liang couldn't help but lean back slightly.
"Hmm~!"

Wang Wentao couldn't stand it first. When he smelled the smell, he couldn't help but scream.

He didn't care that the weather was getting cold, so he could only open the door of the rat room and let the cold air from outside the door blow in, which would dissipate the strange smell in the rat room.

At this time, Wang Wentao asked: "What does this smell like?"

"The smell of half-digested food after fermentation." Lu Liang answered quickly.

Wang Wentao shook his head: "It's really hard to describe."

After scraping the chyme in the stomach, Lu Liang saw a bleeding area on the gastric mucosa of the dead mouse.

really.

After Lu Liang determined the problem, he immediately felt relieved.

At this time, Wang Wentao came up curiously and asked: "Brother, how did this mouse die?"

Lu Liang said while packing up the things on the experimental table: "This mouse was not properly controlled during the gastric administration and the gastric mucosa was scratched. Just be careful next time."

To administer drugs to mice, a syringe is inserted from the mouth of the mouse into the stomach for administration. However, the needle of the syringe is replaced with a special needle that is not as sharp as an ordinary syringe needle.

But if the operation is not done well, the gastric mucosa of the mouse will still be damaged.

After listening to Lu Liang's words, Wang Wentao nodded suddenly, and then he asked in confusion: "The other two, are they also due to gastric administration?"

As before, Lu Liang quickly picked up the scissors and tweezers, completed the dissection of the mouse, checked the mouse's organs and tissues, and then replied: "I'm not sure about these two, the organs and tissues are normal."

"If nothing else happens, he should die."

(End of this chapter)