Rebirth of the God-level Xueba

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Yang Rui slept in the ion channel laboratory for several days.

)

It wasn't that he wanted to overdraw his physical strength to fight from the beginning. However, Su Xiankai, Fan Zhenlong and Wang Sisheng were very motivated, so he had to reluctantly work overtime.

In fact, if you enter the middle of the experiment, the three people working overtime may not really affect him. The scientific research apes are exhausted and the breeder travels around the world to give too many speeches. However, the early stage of the experiment was different. There were so many things that Yang Rui needed to confirm, so that the three of them had so many problems that Yang Rui could not hide.

The work of the project leader is like this. If the Daniel and Mavericks research dogs under his hand are all right, and they are all working step by step, then he can also work step by step, and even take a step by step vacation, just like the rancher is in good weather. The cowboy is strong, and you can enjoy the fresh air, the delicious steak, and the plumpness of the cowgirl or the male cowboy or the sheep when there is nothing to do.

However, the weather is good, the cowboys are strong, and there are too few things around.

Especially for a newly-built ranch, it is simply a matter of rushing in. Just confirming the purchase of reagents, Yang Rui has been busy all afternoon.

If the g-protein coupled receptor group runs smoothly, like buying reagents or something, Yang Rui can completely ignore it. It's just a routine laboratory life, just like what brand of gloves and what type of gloves to buy. It's as easy as drawing paper.

But at the beginning, Yang Rui really couldn't take these small problems lightly.

The simplest reagent purchase is also about compatibility, such as buying domestic reagents or imported reagents. It seems easy to say that if you have money, you can buy imported ones. If you don’t have money, you can buy domestic ones.

For scientific research dogs who graduated in two or three years, maybe the answer is true, but from the point of view of the project leader, the problem is completely different.

What Yang Rui had to determine at the beginning was actually the reagents needed for the entire experimental cycle. Otherwise, if you use imported reagents in the early stage and domestic ones in the later stage, and then compare the two, the result curve will definitely be messy. In this kind of high-standard international scientific research competition, the articles are all directed at s, and the pictures in there are to be read repeatedly or even repeated experiments. The mess will destroy a good data.

In addition, buying reagents is not limited to the difference between imported and domestic products. Yang Rui's final answer on paper is not only to decide which country it is from, but also which company, which factory, and even a certain batch. .

The purity of the reagents can be selected from superior grade, analytical grade, chemical purity, chromatographic purity, etc., which correspond to the amount of impurities. However, for some reagents, no matter how high the standard is, it is still impossible to completely eliminate impurities. For experimentation For people, this is a very annoying thing. You don’t have the conditions. How can I do the experiment?

Unfortunately, it does not mean that scientific research can be done without the actual conditions. The correct way is to ensure the consistency of impurities. The impurities of the same batch of reagents in the same factory are usually guaranteed to be consistent. In this way, compare them. And calculations can eliminate the influence of impurities to a large extent.

Extending the same method, two conclusions can be drawn. First, even if it is a high-purity experimental reagent, it is better to buy a batch, because you don't know if the other party's factory will produce a batch of unqualified reagents. Putting one's own money for several years and millions of dollars into the prudence and professionalism of others is not cautious and unprofessional towards oneself.

Not to mention, scientific research is always doing things with small probability.

The second conclusion is that it is possible to use lower purity reagents to do relatively demanding research, as long as the data is processed later-this is completely in line with the rules, and the result is similar to the experiment made with medium and high purity reagents. . Chinese laboratories have always done this, the main reason is...Of course, it is because of poverty.

In addition, reagents are not as pure as possible.

The first question I face is naturally whether the purse can bear it. If the purse can bear it-in extreme cases, there are actually examples of not buying high-purity reagents. For example, people who make materials often like to buy domestic reagents, because domestic reagents often have them. Inexplicable impurities can occasionally bring surprises to people.

There are many examples of so-called impurity meritorious service in the history of science.

Secondly, the purse is not just buying reagents and looking at expensive ones.

For the concentrated sulfuric acid used to dispose of waste liquid, you can't buy analytically pure ones. Even if you are rich in liquid oil, it is a bit too hard to unpack and fill small-packed analytically pure bottles into the waste liquid bucket.

It's totally thankless.

Besides, those who do scientific research on the earth are not really rich. Take the biological laboratory in 1986 as an example. For a gram of 50 million US dollars of interferon, you would say you want a few tons.

The g-protein coupled receptor was originally a money-burning project. Starting at $6 million, enough is enough, but it must not be wasted arbitrarily, especially in the early stages. It is more careful to establish standards.

You can't open up because of the fog of war, you immediately come to a double base.

Many of the reagents used in the early stage will have to be used all the way to the end of the project. If the project is to be used for three to five years, or even five to eight years, it is impossible to say that in the seventh year of the fourth year, suddenly switch to cheaper reagents. The value of the previous experimental data is greatly reduced. Therefore, instead of gnashing your teeth at that time, you must establish your own standards in the early stage.

This is actually a challenge for Yang Rui.

How to plan the start. There is no one to teach, and there is no fixed number. The world is changing too fast. To some extent, there must be some predictions. This kind of prediction not only predicts the time of the project, but also the expected. Funding also needs to predict the status of the opponent and the trend of the scientific research community.

Of course, the most important thing is experience.

If you are in developed countries such as Europe and America, Yang Rui can also try to consult scholars from other top laboratories. Unfortunately, there are no corresponding conditions in China.

It is Professor Cai and others. Although they have made international-level results, they have not established an international-level laboratory. They have obtained international-level results under the condition of carrying out international-level projects.

In all, since China has synthesized bovine insulin, there has been no such targeted large-scale biological research and development. In the past two to three decades, there have been almost none of the scientific research in biology that has made clear the chariots, prepared for war, and then participated in a positive international level scientific research competition. In terms of speaking, they are almost all ambush special operations or special forces behind enemy lines.

Back in those days, when the Institute of Biochemistry of the Chinese Academy of Sciences, the Department of Chemistry of Peking University, and the Institute of Organic Chemistry of the Chinese Academy of Sciences assembled a heavy army, organized the distribution of jackets, the leaders made pre-war mobilizations, and each handed a scalpel to prepare synthetic bovine insulin. "Nature" is published one after another. Although it is only an American laboratory and a German laboratory that are fighting against the whole country, it is indeed the highest level of confrontation in a specific scientific research field, and it shocked the world. Maybe not. Shocking China did indeed.

However, that was also 30 years ago.

Therefore, Yang Rui has nowhere to consult.

However, from a good point of view, this also leaves Yang Rui no competitors and less expert guidance.

"It's my own money anyway, I pay myself the tuition..." Yang Rui gritted his teeth and signed various documents and projects.

The U.S. dollar account of the People's Bank of China is also declining. Distressed branch business managers all called to inquire.

100,000.

$200,000.

$500,000.

1,000,000.

US$2 million.

By the time the preparatory work was completed and the equipment and consumables to be used in the early stage were ordered, 2.7 million US dollars had been paid out.

Looking at the numbers announced inside the laboratory, Su Xiankai felt bored at the time, and in a flash, he felt a cold sweat on his back.

"Fortunately, you didn't listen to me at the time." Su Xiankai followed from beginning to end, knowing that Yang Rui did not choose particularly expensive products. He basically searched in the lower-medium range, which is a relatively frugal opening mode. It's not that you can no longer be frugal, but if you continue to be frugal, you can only do tracking experiments, and you are not eligible to participate in scientific research competition.

However, with such a standard, it was 2.7 million U.S. dollars, which was almost three times the figure that Su Xiankai wanted. Su Xiankai was also frightened for a while.

There is no turning back at the start of the work, and the $1 million has been spent. If you don’t see the noise, you won’t be able to get it back.

Yang Rui didn't feel fortunate either. He just said: "It doesn't matter if you succeed once, the remaining funds can make people happy, maybe there are one or two seven figures left..."

"Director Yang, don't irritate us. We didn't even find the door." Fan Zhenlong couldn't laugh. Recently, he and Wang Sisheng practiced with Wang Sisheng for a purified g protein, let alone the whole experiment.

Su Xiankai, a skilled worker who has purified a group of protein g, also didn’t have any confidence, and said: "If you can achieve rhodopsin protein, then if there is another success, I already feel a little bit here, but you definitely can’t ask for it. It's so low."

Yang Rui rolled his eyelids and said, "Bovine rhodopsin protein is the experimental material, and it hasn't been included in the experimental procedure yet, Lao Fan, where is your side? Is the detergent useful?"

Detergents can increase the solubility of protein g. It is the best method for purifying protein g. It is also a secret book in high-end laboratories and has not been widely disseminated.

But Fan Zhenlong still shook his head and said, "Easy to use is easier to use, but we have to extract the receptor from the cell membrane. We haven't found the trick yet. Lao Su doesn't understand it either."

Yang Rui looked at Su Xiankai naturally.

Su Xiankai was confident, and said: "Lao Fan and the others chose to purify GT. I haven't touched it. I start over again. I can't grasp the various dosages. I actually don't have any tricks. I just do more. This is done. It was only purified in one year."

There are many types of g-protein and g-protein coupled receptors, and a certain amount is necessary to make a systematic comparison. Therefore, although purification is for Fan Zhenlong and others to practice hands, it is also a necessary condition in the early stage of the experiment. One.

But if you have to wait for another year and a half ~www.readwn.com~ to have one more purified protein, the laboratory can't afford to wait.

Yang Rui couldn't help lowering his head in thought.

Using detergent to purify g protein, this secret is what he reads from the book, and he has not operated it on the spot. Moreover, as far as his level of **** experimentation is concerned, there is no specific experimental guidance.

In any case, Fan Zhenlong and other Mavericks' experimental level is better than him. However, they will become great in the future, instead of relying on their experimental level. In terms of experimental level alone, the three of them are afraid that Gu Qiang will not have fun.

Thinking of this, Yang Rui couldn't help but patted his forehead and said, "In this way, I will add another farmer to you... No, add an experimental assistant, Gu Qiang from the genetic engineering laboratory. He has a strong hands-on ability and you cooperate with each other. a bit."

Learning also needs help. Since the speed of idle mining is slow, just add a farmer.

Anyway, the genetic engineering laboratories are also under his name, and they are all public laboratories. It is very convenient to borrow something.

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