I Have A Super USB Drive

Chapter 20: Experimental process

   half a month later.

  Chen Chen stopped the instrument in his hand and stretched out in front of the hundred-level ultra-clean workbench, a look of excitement flashed in his eyes.

   "The induction of the fertilized egg of clone 30 is completed and it can be implanted inside the mother.

   Chen Chen started to fill in the experimental records as he stated. The experimental recorder worn on his head faithfully records every frame of the picture. These records will become the most precious data in the future.

   At this time, the entire experiment is still in the early stage, that is, the cloning stage. Chen Chen needs to use a micromanipulator to remove a total of 36 egg cells from the nucleus and implant another nucleus to form a cloned fertilized egg.

   This is also commonly known as "somatic cell nuclear transfer reprogramming."

The journey of cloning technology is not smooth. From the first idea of ​​cloning by German embryologists in 1938, to the birth of cloned sheep Dolly in 1996, and then in 2012, humans discovered that caffeine can increase the success rate of reprogramming of primate egg cells. , Human cloning technology has been developed for nearly a century.

   Until today, cloning technology has matured and commercialized. Many people are willing to spend a lot of money to clone dead pets and the emergence of profitable pet cloning companies are enough to show that cloning technology has matured day by day.

   Because of this, Chen Chen was able to process dozens of SD rat cloned fertilized eggs within half a month.

   However, mature technology does not mean simplicity.

  What is clone?

   There was such a fable in the West:

   said that there is a country where there is a toxic spring water. Anyone who takes a sip will go crazy and become a lunatic.

   All the subjects of this country drank this spring water and fell into a state of madness, but the king did not drink it, so he did not go crazy with it.

  As a result, the lunatics all over the country felt that the king had mental problems, so they **** the king and tried to treat his madness.

  In the end, the king couldn't stand the torture, so he could only take the initiative to drink the spring water and be crazy with the people...

  Clone is the true reflection of this fable.

   In a cell, there are dozens of materials such as cell membrane, cytoplasm, organelle and cell nucleus. The most important of these is the cell nucleus, which is an organ that contains all the genetic information in the cell.

Generally speaking, in order for a cell to perform activities, it must first declare to the nucleus. After the nucleus is approved, the relevant genes will be called, and then a molecule called messenger RNA will be used to carry out the genetic information so that the relevant organelles can perform their own command.

   It can be said that in the cell, the nucleus is the king, and the other structures and components in the cell are the subjects.

   But if one day, scientists replace the king of cell nuclei, especially egg cells, humans have removed the nucleus of the egg cell, and stuffed the nucleus of the somatic cell of the object to be cloned into the egg cell instead.

   Then, scientists used electric current and chemical agents to change the characteristics of this egg cell, turning it into a fertilized egg, just like letting the whole country drink poisonous spring water, so a scene in the fable happened.

The subjects asked the nucleus to give orders for the development of the fertilized egg, but the newly appointed nucleus refused it. After all, he is not the nucleus of the egg cell. If you can’t let me develop the fertilized egg, I will develop. My somatic cell nucleus is a mutation. It becomes cancerous, swallowed by white blood cells, and never develops!

   However, with the continuous pressure of the subjects, this somatic cell nucleus finally felt "unfortunate for public anger" and had to change its position to transform itself into an egg cell nucleus.

   At this point, the egg cell has successfully developed into an embryo, which will be implanted into a mother's uterus, and a cloned life will eventually be born.

   And in this life, there is exactly the same gene as the owner of the nucleus flowing in the body.

   Successfully cloned!

   Of course, the actual operation is still very difficult. Not to mention the damage of the chemical agents, just replacing the nucleus on the egg cell is equivalent to a major operation.

   After all, the egg cell needs to remove the cell nucleus first, and then implant the allogeneic cell nucleus. The egg cell is already dead if it is not killed on the spot.

   Chen Chen, who also took NZT48, can maintain such an efficient success rate.

   At this moment, the door behind Chen Chen was knocked, and Wang Wei's voice came in.

   "Chenzi, the second batch of clones were successfully born, and no hidden diseases have been found for the time being."

   Unlike Chen Chen, who is full of energy, Wang Wei's voice is unconcealable fatigue.

   "Okay, I'll come right now."

   Chen Chen stood up and began to clean up the workbench in front of him. After freezing a few precious cloned fertilized eggs, he hurried to another room.

   As soon as he entered the door, Chen Chen heard a squeak. It was the calls of several newly born rats, while Wang Wei and Xia Yin were surrounded by the cage and silently observed.

   The whole body of the newborn rat was red, with only two black spots as large as sesame seeds in the eyes, squeaking like a red worm.

   "Get ready to start, extract neural stem cells to make cell suspension."

"it is good."

  Wang Wei nodded. After a month of working together, Wang Wei has been completely convinced by Chen Chen's skill and perseverance. If it weren't for Chen Chen, no student in the entire Academy of Biological Sciences could do this.

   Xia Yin on the side opened her mouth and looked at a bunch of newly born rats that did not even open their eyes, showing hesitation, "Are we really going to do it now?"

   "Since you have chosen this line, don't feel pity." Chen Chen said lightly, "It is enough to maintain respect for this group of animals dedicated to science."

   "..." Xia Yin was silent.

   Immediately, the three of them began to compare the cloned rat with the original owner, and after confirming that there was no error, they were put to death.

   The poor newborn rat has completely lost his life before he even opened his eyes to see the world.

   "Is the ice PBS dish ready?" Chen Chen asked.

   "Here." Xia Yin carefully brought a few petri dishes and looked at Wang Wei, "It's time to start."

"it is good."

  Wang Wei took the petri dish and began to execute the pups on the operating table, and took out the brain tissue from the corpses of the pups.

Subsequently, he placed the rat’s brain in an ice PBS dish, and skillfully separated the hippocampus, put it in buffer, and then it was like an assembly line, UUReading www.uukanshu.com turned around again to process the second rat .

   Chen Chen walked to the place where Wang Wei was before, cut the hippocampus into fine pieces, and added 0.25% pancreatin at the same time, then put it in a 37 degree incubator, and said to Xia Yin, "Is the planting liquid ready?"

   "Ready." Xia Yin took the test tube from the shelf.

   Chen Chen nodded and turned around to start the operation on the second petri dish that Wang Wei had processed.

   Xia Yin waited for two minutes, then took out the petri dish from the incubator, put it in the centrifuge for separation, and then added the planting solution and pipetted to form a cell suspension.

   "Didididi!"

   Just then, the electronic alarm clock in the laboratory rang, and it was nine o'clock in the evening.

   "Well, let's end the experiment for today."

  Although there is still unfinished work, Chen Chen didn't mean to stop at all. He put down the tools in his hand, cleaned up the things, and turned and walked outside the laboratory.

   "But we still have half an hour to finish today's work?" Wang Wei puzzled.

   "Work and rest are the most important." Chen Chen shook his head and solemnly said, "If you want to do this business for a long time, you can't have a good body."

   Watching Chen Chen leave, Wang Wei and Xia Yin looked at each other.

   "Chen Chen is really strange."

   Xia Yin vomited, “Working race against time and full of energy, one person can do the workload of several people, but once it is the off-get off work time set by himself, he is unwilling to do one more second...

   "Maybe this is the quirk of scientists?"

  Wang Wei shook his head, "Let's clean up too, and we will do the rest tomorrow."

"it is good."

   Xia Yin nodded, and the two left the laboratory one after another.

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