Xueba’s medical black technology system

Chapter 199: Sampling is completed and the animal experiment is over.

The experiment was carried out relatively smoothly.

The next step is a more repetitive task, which is to administer gavage to the mice.

The drug was injected into the stomach of mice at a fixed dose once a day.

However, after learning that the price of these mice was 500 yuan each, Wang Wentao became cautious.

I was afraid that I would kill these mice accidentally.

Lu Liang glanced at Wang Wentao's careful actions and said: "Be bold, there is no need to be so cautious, mice are not so fragile."

After listening to Lu Liang's words, Wang Wentao became a little bolder at this time.

After completing the weighing, Lu Liang entered all the weight data into the computer.

Wang Wentao asked: "Brother, you said these mice are so expensive. If the experiment fails, wouldn't it be a loss?"

Wang Wentao's idea is also very simple.

Generally speaking, if the research team wants to publish an article, experiments are definitely indispensable.

The experiment process is often accompanied by failure.

Ji Hong's scientific research funds are now abundant, so he can create them casually, but the scientific research funds of other professors are not so abundant.

If this experiment fails, tens of thousands of dollars will be lost. Which professor can afford this?

Lu Liang replied: "So, other research groups will choose to buy ordinary mice and then induce liver cancer models in the mice themselves."

"What do you mean, senior brother?" Wang Wentao asked curiously. He has not designed experiments yet, so he doesn't know much about this aspect.

Lu Liang explained to Wang Wentao: "Take the liver cancer model as an example. Under normal circumstances, I should first buy a batch of normal mice, and then pass some carcinogenic reagents, such as diethylnitrosamine, aflatoxin, Carbon tetrachloride and so on.”

"By using a sufficient dose of a carcinogen, liver cancer can be induced in mice over a period of time."

"This is the most common method of culturing mice for liver cancer models."

Lu Liang continued: "I don't have time to cultivate a mouse model of liver cancer, so I buy it directly from a biological company, so the price is relatively expensive. If it were just an ordinary mouse, the price would not be so outrageous."

Wang Wentao suddenly understood and nodded.

Lu Liang seemed to have remembered something: "However, when you use these reagents in the future, you must take precautions."

Due to experimental factors, many reagents in the laboratory are extremely carcinogenic.

Acid preparations, fixatives, WB reagents, inducers used to induce mouse models, etc.

Especially those inducers used in mouse modeling.

Since these reagents can induce cancer in mice, they can also induce cancer in humans.

For students, they will inevitably come into contact with these highly carcinogenic reagents from the moment they step into the laboratory.

If the instructor is conscientious, he may prepare enough gloves and masks in the laboratory, or replace the laboratory with some more expensive but safer reagents.

But unfortunately, not all teachers have a conscience.

In the eyes of some unscrupulous tutors, students are just consumables that can be thrown away after use.

Not to mention protective equipment, even cheap disposable rubber gloves or disposable masks often require using them two or three times before throwing them away.

Even when faced with volatile reagents, students are even more vulnerable.

But even if these mentors knew this, they wouldn't care too much.

Because these people care more about how much money they can save than the students' health.

After all, academic qualifications can only screen a person's cognitive level, but they cannot screen a person's moral level.

Lu Liang threw the last mouse into the cage, and today's work was considered completed.

After Lu Liang cleaned up the rat room, he called Wang Wentao to prepare for dinner.

In the following time, Lu Liang and Wang Wentao basically maintained a three-point-one-line life of dormitory, study room, and mouse room.

And time flies so fast, more than 20 days go by in a blink of an eye.

Lu Liang counted with his fingers and found that the end date of the trial was coming soon.

So Lu Liang began to prepare a series of sampling items such as liquid nitrogen, centrifuge tubes, and paraformaldehyde.

At the same time, we also made an appointment with Wen Yong, Shang Peng and others to come over and help with sampling.

After all, just two people, Lu Liang and Wang Wentao, might not be able to complete the sampling of all mice from morning to night.

Naturally, Wen Yong and Shang Peng agreed unequivocally.

On the day of sampling, a group of people came to the rat room early in the morning.

Wen Yong and Shang Peng could be said to have called on all the people in their research group. In total, there were seven or eight people in total.

Wen Yong asked: "How many mice are there this time?"

Lu Liang replied: "About a hundred or so."

Wen Yong thought for a moment and then replied: "It's quite a lot. If everyone works harder, let's try to sample all the mice in one morning." Under Lu Liang's call, everyone started to take action.

Wen Yong and others are still very motivated. After all, they know very well that as long as they contribute to the experiment, Lu Liang will not treat them badly.

Even if it doesn't work, Lu Liang will transfer some labor fees to them.

Soon, everyone started to get busy.

There are not many samples that need to be taken this time, focusing on serum, liver and kidney samples.

Lu Liang was responsible for collecting blood from the mice, while Wen Yong and Shang Peng were responsible for dissection, and the rest were responsible for sampling.

Everyone in the entire mouse room works step by step, like a huge assembly line, and the mice are the products to be processed on the assembly line.

As the assembly line started running, one by one the mice embarked on the road of dedicating themselves to science.

Soon, all the mice in the control group were dissected, and then it was the turn of the mice in the experimental group.

After dissecting the first mouse, Shang Peng immediately noticed something was wrong: "The lesions on the liver of this mouse have changed a bit obviously?"

Shang Peng said while playing with the liver with tweezers. The lesions he was referring to were tumor tissue on the mouse liver.

"What, is there any problem?" Lu Liang asked from the side.

Shang Peng nodded and said: "Compared with the livers of mice in the control group, the liver lesions of the mice in the experimental group have shrunk a lot, and the number has also become smaller."

Wen Yong on the side also joined in at this moment and said: "Would you like to take some photos and compare the results with the photos?"

Photos of these tumor tissues can also be included in the paper, because this is also a form of expression of the results.

Moreover, results expressed through photos are often more intuitive and convincing than data.

However, Lu Liang frowned and said, "No, if you use photos, it might not be obvious."

The reason why Wen Yong looks obvious is because he is at the scene and can see it with the naked eye.

But if you change it to a photo, the comparison may give people a different feeling.

"What about using tumor tissue slices to get the results?" Wen Yong asked tentatively.

"The sections of the tumor tissue are only part of the results." Lu Liang replied with a smile.

This confused Wen Yong and Shang Peng.

In addition to sections of tumor tissue, are there other related sections that can be done?

Lu Liang replied: "I plan to make a slice of vascular tissue at that time."

After hearing Lu Liang's answer, both Wen Yong and Shang Peng looked puzzled.

Wen Yong said: "Isn't this unnecessary? Even if the blood vessel tissue slices are made and there are differences, they can't prove anything."

Lu Liang shook his head and explained: "Think about it, since my drug targets anti-angiogenesis.

So under the inhibition of the drug, there must be a difference in the inner diameter of the blood vessels in the tumor tissues of the mice in the control group and the mice in the experimental group. "

"After being inhibited by drugs, the number of blood vessels in the mouse tumor tissue will be less, and the inner diameter of his blood vessels will be very small, which will form a very obvious contrast."

If this slice is made and the result is in line with Lu Liang's expectations, then this slice can largely prove the effect of his drug.

After hearing Lu Liang's thoughts, Wen Yong and Shang Peng suddenly became enlightened.

Wen Yong couldn't help but smacked his lips: "Brother Lu, your thinking is a bit tricky!"

Several people exchanged various ideas while working.

Finally, near noon, all mice had been dissected and sampled.

After Lu Liang cleaned up the rat room, he called Wang Wentao over: "You will take everyone out to have a meal together later, and I will transfer the money to you at that time."

Not to mention that it is an old tradition among all research groups to treat someone to a meal after helping with the experiment.

Secondly, if others work hard to help with the experiment until noon, if they don’t treat them to a meal and let them go back hungry, there will always be some grudge in their hearts.

If you ask others for help by then, I'm afraid no one will be willing to come and help.

Those who engage in research should also be a little more sophisticated.

Wang Wentao nodded, and then asked: "Brother, aren't you going to eat?"

Lu Liang said: "I don't have time right now. I have to go back to the laboratory first and centrifuge the serum."

Now the blood in the centrifuge tube is whole blood. If it is left for a long time, some indicators in the serum will change, and then this batch of serum will be completely useless.

You should know that most of the experimental data are obtained by detecting serum.

Without the serum, the entire experiment would have been essentially in vain.

Therefore, he needs to centrifuge the serum as quickly as possible and then store it in the refrigerator.

Wang Wentao nodded: "Okay, senior brother, I'll leave first."

After Wang Wentao, Wen Yong and others left, Lu Liang put away the centrifuge tubes filled with blood, then took his things and walked towards the laboratory.

(End of this chapter)