Xueba’s medical black technology system

Chapter 119: Put some pressure on Lu Liang

“分别加入0、100、200、500、800、1000umol/L的索非布韦/维帕他韦，0作为空白对照组，随后每孔加入100uL细胞培养液。”

"Discard the culture medium, add 150uLDMSO solution to each well, and place on a shaker at 100 r/min to avoid light."

"Finally, adjust the spectrum to a wavelength of 570nm on the microplate reader for detection."

In the laboratory, Lu Liang quickly placed the 96-well plate on the microplate reader. As the 96-well plate was swallowed into the machine, a buzzing sound came from the machine.

Then data quickly appeared on the computer screen one by one.

Lu Liang was not in a hurry to copy the data to his USB flash drive. He pulled out an excel table and then established a standard curve on the table.

R = 0.999
The standard curve is correct.

Lu Liang put all the data into the formula for calculation, and then pulled out the average and variance.

"From the data point of view, there is not much fluctuation in sofosbuvir/velpatasvir at 100, 200 and 500umol/L, and it seems that it does not have much impact on liver cell proliferation."

"With 800umol/L sofosbuvir/velpatasvir, the data difference is beginning to be somewhat obvious. Excessive drug dosage will still affect the proliferation of liver cells."

"Not to mention 1000umol/L of sofosbuvir/velpatasvir, the liver cells are almost dead."

Lu Liang looked at the data and thought about it.

The final result of most experiments is to pursue one thing, and that is significance.

Significant means there is a difference, it means there is a change, indicating that your stuff works.

Not significant, no difference, which means it’s the same with or without you.

But sometimes, being subtle is just as important.

For example, now, the current test is to detect whether the drug affects the proliferation of liver cells.

The results of this experiment were naturally not significantly better.

The results were not significant, indicating that after the drug is taken, it will not cause much damage to the liver cells in the liver.

If this data is significant, then it will be really troublesome.

Once the medicine is taken, the liver cells will be completely dead, let alone the effect on the virus.

"It seems that the drug does not have much impact on liver cells at low concentrations, but once the concentration is too high, it will still inhibit the proliferation of liver cells to some extent."

Looking at the data on the screen, Lu Liang came to a rough conclusion in his mind.

Although these data have not been analyzed by SPSS professional software, as long as you are sensitive enough to numbers, you can still see some clues.

Since there are no problems in the first part of the test, the next step is to see how the drug affects the replication of viral mRNA.

Lu Liang glanced at the time. It was now 12 noon. After dinner, he could start the most critical experiment.

After packing up his things, Lu Liang left the laboratory.

However, Lu Liang had just walked to the door of the study room when he heard a familiar voice coming from the study room, the voice mixed with complaints and abuse.

"Why is this Ruimai Pharmaceutical so shameless? How can you blame others for this kind of thing?"

The door of the study room was pushed open, and Liu Yao walked out of the study room. After bumping into Lu Liang, Liu Yao said hello: "Have you finished your morning experiment?"

Lu Liang nodded: "Well, prepare to continue after eating."

"It just so happens that I'm going to the cafeteria too, let's come together."

The two of them walked downstairs, and Lu Liang asked: "What were you scolding just now?" When Lu Liang asked, Liu Yao seemed to have found an outlet, and her full of turmoil and anger spewed out: "Then Jia Ruimai Pharmaceutical published an article, and the people who read the article had high blood pressure."

"What article?"

"It's on the scarf. You'll know by looking at it yourself."

Lu Liang took out his phone out of curiosity and opened his scarf curiously. Soon he found the article Liu Yao mentioned under the official scarf of Remai Medicine.

The article first introduced everyone to the science of hepatitis C, and then logically mentioned the hepatitis C treatment drug Bailize.

From here on, the flavor of the article begins to change.

Remai Pharmaceuticals described at length how it worked tirelessly to negotiate in order to obtain the patent authorization for the Bailize drug and to provide treatment drugs for the majority of hepatitis C patients in China.

But in the end, it seemed that the negotiation was about to succeed, but because of Lu Liang, all the previous efforts were in vain.

In the article, Remai Medical directly portrayed itself as a victim, and then pointed all the fingers at Lu Liang, accusing Lu Liang of not considering domestic patients at all.

Lu Liang glanced at the article briefly, and then shook his head. It was such a boring trick again. Besides writing articles, just writing articles, couldn't he do some other innovative tricks?

"However, this time the public's eyes are sharp. Everyone in the article is criticizing Remai Medical." Liu Yao said.

This somewhat surprised Lu Liang, and he quickly opened the comment area.

"Can you please have some shame? If you can't poop, it's the toilet. If you don't have the ability, why should you blame others?"

"If you can't get the patent authorization, can't you develop new drugs by yourself? After all, you are still a pharmaceutical company."

"Let it go, let it go. This kind of pharmaceutical company is hopeless. It can only write short essays when it encounters problems. It deserves to be choked."

Sure enough, just as Liu Yao said, the comment area was full of abuse against Remai Medical.

Lu Liang's expression suddenly became strange. He estimated that Wang He himself did not expect this to happen.

After all, Wang He did this to put some pressure on Lu Liang, but he didn't expect that the pressure would be on him.

This also gave Lu Liang a sense of urgency.

Although he knew that Wang He would definitely not be so peaceful, he didn't expect it to come so quickly.

Moreover, even if Wang He failed this time, he probably would not give up, and there would probably be some small moves in the future.

However, as soon as his experimental data comes out, Wang He will not be able to get up.

Thinking of this, Lu Liang narrowed his eyes slightly. It seemed that he had to hurry up with his experiments.

Liu Yao asked at this time: "How many experiments do you have left to complete?"

Lu Liang thought for a moment and replied: "The last part is still left, it depends on how well the drug inhibits the replication of the virus strain."

The next thing to do is to detect the level of hepatitis C virus mRNA in cells after drug treatment by fluorescence quantitative PCR.

This is equivalent to a simulation that simulates the suppression of the virus by the drug after a person takes the drug.

Because there are six different genotypes of hepatitis C virus, the test needs to be repeated for each genotype of the virus strain.

In other words, as long as this part of the test is completed, the entire test is almost over.

Liu Yao next to her nodded in understanding.

"Okay, let's not talk anymore. I'm going back to the laboratory first." Lu Liang finished the last bite of food on the plate, and then hurriedly walked towards the life science building.

(End of this chapter)